FIG 5.

LegC4 enhances IFN-γ-mediated restriction of L. pneumophila replication. (A) Fold replication of the indicated L. pneumophila strains within wild-type (WT) BMDMs in the presence of 50 ng ml⁻¹ rIFN-γ. (B) Fold replication (48 h) of the indicated strains within wild-type BMDMs in the presence or absence of 5 ng ml⁻¹ rIFN-γ. (C) Fold replication of the indicated L. pneumophila strains within Tnfr1^−/− BMDMs in the presence of 50 ng ml⁻¹ rIFN-γ. Expression of legC4 was induced with 1 mM IPTG. Data shown are mean ± SD for samples in triplicate. Asterisks denote statistical significance (**, P < 0.01; n.s., not significant) by Student’s t test. Data are representative of at least two independent experiments.