TABLE 2.
Effects of metabolic compounds on GDH activity
| Compound | TtGDH APRTha
|
TtGDHb
|
||
|---|---|---|---|---|
| Reductive amination (%) | Oxidative deamination (%) | Reductive amination (%) | Oxidative deamination (%) | |
| None | 100 ± 2 | 100 ± 1 | 100 ± 4 | 100 ± 1 |
| AMP | 626 ± 19 | 252 ± 1 | 98 ± 2 | 104 ± 3 |
| ADP | 152 ± 6 | 120 ± 1 | ND | ND |
| ATP | 124 ± 5 | 111 ± 5 | ND | ND |
| GMP | 133 ± 6 | 101 ± 1 | ND | ND |
| GDP | 113 ± 9 | 103 ± 1 | ND | ND |
| GTP | 108 ± 15 | 105 ± 6 | ND | ND |
| IMP | 116 ± 5 | 100 ± 1 | ND | ND |
| Leu | 2,440 ± 134 | 335 ± 3 | 459 ± 14 | 432 ± 2 |
| Leu/AMP | 5,670 ± 102 | 457 ± 6 | 470 ± 14 | 426 ± 1 |
a
100% denotes 0.60 U/mg and 3.5 U/mg of TtGDH-APRTh for reductive amination and oxidative deamination, respectively.
b
100% denotes 3.7 U/mg and 5.4 U/mg of TtGDH for reductive amination and oxidative deamination, respectively. ND, not determined.