TABLE 3.
Strains used in this study
| Strain | Genotype or description | Source or reference |
|---|---|---|
| E. coli | ||
| OmniMAX-2 T1R | F′ [proAB+ lacIq lacZΔM15 Tn10(Tetr) Δ(ccdAB)] mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80(lacZ)ΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD | Invitrogen |
| HB101/pRK24 | F− mcrB mrr hsdS20(rB− mB−) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20 | 49 |
| C. difficile | ||
| R20291 | Wild-type C. difficile strain from UK outbreak (ribotype 027) | |
| 630Δerm | Spontaneous erythromycin-sensitive derivative of strain 630 (ribotype 012) | 55 |
| CRG1496 | 630Δerm ΔpyrE | 13 |
| UM275 | 630Δerm with Pveg::rfp downstream of pyrE | This study |