TABLE 4.
Plasmids used in this study
| Plasmid | Relevant feature(s) | Reference |
|---|---|---|
| pJK02 | Ptet::Cas9-opt Pgdh::sgRNA colE1 CD6 ori RP4oriT-traJ catP | 12 |
| pJMP2 | Pveg::sgRNA colE1 catP amp spc | 23 |
| pJMP4 | Pveg::rfp colE1 catP amp spc | 23 |
| pRPF185 | E. coli-C. difficile shuttle vector with a tetracycline-inducible promoter; Ptet::gusA tetR CD6ori RP4oriT-traJ pMB1ori catP | 7 |
| pdCas9_bacteria | Ptet::dCas9 tetR ori15A catP | 20 |
| pMTL-YN1C | E. coli-C. difficile shuttle vector for inserting genes into the C. difficile chromosome while restoring pyrE; colE1 RP4oriT-traJ CB102ori-repH′ catP | 13 |
| pDSW1728 | Ptet::mCherryOpt catP | 51 |
| pDSW1963 | Pveg::rfp catP | This study |
| pIA17 | Pveg::rfp in pMTL-YN1C catP | This study |
| pCE531 | Ptet::dCas9 catP | This study |
| pIA18 | Ptet::dCas9 PsigA::sgRNA-neg catP | This study |
| pIA19 | PxylB′::mCherryOpt catP | This study |
| pAP114 | Pxyl::mCherryOpt catP | This study |
| pIA20 | PxylB′::dCas9 PsigA::sgRNA-neg catP | This study |
| pIA21 | Pxyl::dCas9 PsigA::sgRNA-neg catP | This study |
| pIA24 | Pxyl::dCas9 Pveg::sgRNA-neg catP | This study |
| pIA25 | Pxyl::dCas9 Pgdh::sgRNA-neg catP | This study |
| pIA26 | Pxyl::dCas9 PsigA::sgRNA-rfp catP | This study |
| pIA27 | Pxyl::dCas9 Pveg::sgRNA-rfp catP | This study |
| pIA28 | Pxyl::dCas9 Pgdh::sgRNA-rfp catP | This study |
| pIA33 | Pxyl::dCas9-opt Pgdh::sgRNA-rfp catP | This study |
| pIA34 | Pxyl::dCas9-opt Pgdh::sgRNA-neg catP | This study |
| pIA35 | Pxyl::dCas9-opt Pgdh::sgRNA-ftsZ-1 catP | This study |
| pIA36 | Pxyl::dCas9-opt Pgdh::sgRNA-ftsZ-2 catP | This study |
| pIA37 | Pxyl::dCas9-opt Pgdh::sgRNA-ftsZ-3 catP | This study |
| pIA38 | Pxyl::dCas9-opt Pgdh::sgRNA-slpA-1 catP | This study |
| pIA39 | Pxyl::dCas9-opt Pgdh::sgRNA-slpA-2 catP | This study |
| pIA40 | Pxyl::dCas9-opt Pgdh::sgRNA-pbp-1 catP | This study |
| pIA41 | Pxyl::dCas9-opt Pgdh::sgRNA-pbp-2 catP | This study |