Figure 1. Effects of brief treatment with cytotoxic drugs on the phenotype of MCF7 breast cancer cells. Colony formation (A), cell migration (B) and invasion (C) assays were performed in MCF7 cells treated briefly with paclitaxel (30 nM) for 1 hour, doxorubicin (200 nM) for 3 hours, or vehicle control. (D) Invasion assay of MCF7 cells treated with paclitaxel (30 nM) for 1 hour, doxorubicin (200 nM) for 3 hours, or a combination of both in different sequences.

^*p < 0.05 versus control; ^†p < 0.01; ^‡p < 0.001.