Figure 3. Effects of brief treatment with cytotoxic drugs on the expression of cell motility factors and RhoA activity in MCF7 breast cancer cells. (A) Cells were treated with paclitaxel (30 nM) for 1 hour or doxorubicin (200 nM) for 3 hours. The media were replaced, and cells were maintained in standard culture medium further for 24 to 48 hours. Cell lysates were prepared and western blotting was performed. (B) Cells were treated with paclitaxel (30 nM) or doxorubicin (200 nM) for 30 minutes. Cell lysates were prepared and RhoA activity was determined.

IKK = IκB kinase; MLC = myosin light chain.

^*p < 0.01 versus control.