Figure 4. Rescue effects of RhoA inhibitors on cell migration and invasion promoted by doxorubicin in breast cancer cells. MCF7 (A, C) and BT-474 (B, D) cells were pretreated with or without Y16 (50 μM) or rhosin/G04 (50 μM) for 24 hours and incubated with doxorubicin (MCF7, 200 nM; BT-474, 800 nM) or vehicle control for an additional 3 hours. Then, cells were re-suspended and migration and invasion assays were performed.

^*p < 0.05 versus control; ^†p < 0.01; ^‡p < 0.05 versus doxorubicin only; ^§p < 0.01.