Figure 1. Upregulation of CDCA8 in Tam-resistant BC cells. (A, B) MCF-7, MCF-7/TR, T47D, and T47D/TR cell proliferation was determined by the MTT assay after 4OH-Tam treatment for 96 hr. (C) Quantitative reverse transcription-polymerase chain reaction analysis of CDCA8 mRNA levels in BC cells. Glyceraldehyde-3-phosphate dehydrogenase served as loading controls. (D) Western blotting analysis of CDCA8 levels in BC cells. β-actin served as loading controls. Data are presented as mean ± standard deviation from three independent experiments.

CDCA8 = cell division cycle associated 8; BC = breast cancer; Tam = tamoxifen.

^*p < 0.01.