REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Anti-FLAG M2 | Sigma | Cat# F1804; RRID:AB_262044 |
Anti-FLAG-HRP | Sigma | Cat#A8592; RRID:AB_439702 |
Anti-HA 3F10 | Roche | Cat# 11867423001; RRID:AB_2314622 |
Anti-HA-HRP | Roche | Cat# 12013819001; RRID: AB_390917 |
Anti-Actin | Millipore | Cat# MAB1501; RRID:AB_2223041 |
Anti-Mouse-HRP | BioRad | Cat#1706516 |
Bacterial and Virus Strains | ||
E. coli ccdB survival | Invitrogen | A10460 |
E. coli TOP10 | Invitrogen | C404003 |
Agrobacterium tumefaciens strain GV3101 | N/A | N/A |
Agrobacterium tumefaciens strain C58 | N/A | N/A |
Chemicals, Peptides, and Recombinant Proteins | ||
Hygromycin B | Invitrogen | Cat# 10687010 |
Glufosinate ammonium (BASTA) | Sigma | Cat#45520 |
PMSF | Sigma | Cat# P7626 |
Complete EDTA-free Protease Inhibitor | Roche | Cat# 11836170001 |
Phosphatase Inhibitor 2 | Sigma | Cat#P5726 |
Phosphatase Inhibitor 3 | Sigma | Cat#P0044 |
MG-132 | Peptides International | Cat#3175-v |
Protein G Dynabeads | Invitrogen | Cat# 10004D |
GlycoBlue | Invitrogen | Cat# AM9515 |
D-Luciferin, potassium salt | Biosynth AG | Cat#L-8220 |
Critical Commercial Assays | ||
Gateway BP clonase enzyme mix | Invitrogen | Cat#11789020 |
Gateway LR clonase enzyme mix | Invitrogen | Cat#11791100 |
ProQuest™ Two-Hybrid System | Invitrogen | Cat#PQ1000101 |
TnT® SP6 High-Yield Wheat Germ Protein Expression System | Promega | Cat#L3260 |
DC Protein Assay | BioRad | Cat#5000116 |
Supersignal West Pico, Dura, and Femto substrates | Pierce | Cat#34577, 34075, 34095 |
iScript cDNA synthesis kit | BioRad | Cat#1708890 |
Maxima SYBR Green qPCR Master Mix | Thermo Scientific | Cat#K0253 |
Ovation® Ultralow V2 kit | NuGEN | Cat# 0344NB-A01 |
Deposited Data | ||
ChIP-seq data | This paper | GSEXXXXX |
Experimental Models: Organisms/Strains | ||
Col-0 | N/A | N/A |
gi-2 | Fowler et al., 1999 | N/A |
GIox | David et al., 2006 | N/A |
PIF5-HA | Lorrain et al., 2008 | N/A |
PIF4-Flash | Pedmale et al., 2016 | N/A |
pif3-1 | Kim et al., 2003 | SALK_030753 |
pif4-101 | Lorrain et al., 2008 | Garlic_114_G06 |
pif5-1 | Fujimori et al., 2004 | SALK_087012 |
pifQ | Leivar et al., 2008 | N/A |
CCA1::LUC | Salome and McClung, 2005 | N/A |
gi-2;pif3-1 | This paper | N/A |
gi-2;pif4-101 | This paper | N/A |
gi-2;pif5-1 | This paper | N/A |
gi-2;pif4-101;pif5-1 | This paper | N/A |
GI-YPET-Flag | This paper | N/A |
pGI::GI-YPET-Flag | This paper | N/A |
PIF3-ECFP-HA | This paper | N/A |
pPIF3::PIF3-ECFP-HA | This paper | N/A |
PIF3-ECFP-HA;GI-YPET-Flag | This paper | N/A |
PIF3-ECFP-HA;gi-2 | This paper | N/A |
pGI::GI-YPET-Flag;pPIF3::PIF3-ECFP-HA | This paper | N/A |
PIF5-HA;GI-YPET-Flag | This paper | N/A |
pCCA1::LUC, gi-2;pif3-1 | This paper | N/A |
pCCA1::LUC; gi-2;pif4-101 | This paper | N/A |
pCCA1::LUC; gi-2;pif5-1 | This paper | N/A |
pCCA1::LUC; gi-2;pif4-101;pif5-1 | This paper | N/A |
pCCA1::LUC; PIF3-ECFP-HA | This paper | N/A |
pCCA1::LUC; PIF5-HA | This paper | N/A |
pCCA1::LUC; PIF4-Flash | This paper | N/A |
Oligonucleotides | ||
Primers used in this study, see Table S2 | ||
Recombinant DNA | ||
pD32-GI | This paper | N/A |
pD22-PIF1 | Pruneda-Paz et al., 2014 | N/A |
pD22-PIF3 | Pruneda-Paz et al., 2014 | N/A |
pD22-PIF4 | Pruneda-Paz et al., 2014 | N/A |
pD22-PIF5 | Pruneda-Paz et al., 2014 | N/A |
pTNT-HA | Chory lab | N/A |
pTNT-Flag | Chory lab | N/A |
pTNT-HA-GI | This paper | N/A |
pTNT-Flag-PIF1 | This paper | N/A |
pTNT-Flag-PIF3 | This paper | N/A |
pTNT-Flag-PIF4 | This paper | N/A |
pTNT-Flag-PIF5 | This paper | N/A |
pTNT-Flag-GFP | This paper | N/A |
pTNT-Flag-P3-1 | This paper | N/A |
pTNT-Flag-P3-2 | This paper | N/A |
pTNT-Flag-P3-3 | This paper | N/A |
pTNT-Flag-P3-4 | This paper | N/A |
pB7m34GW | University of Ghent collection | https://gateway.psb.ugent.be |
pB-35S::GI-YPET-Flag | This paper | N/A |
pB-pGI::GI-YPET-Flag | This paper | N/A |
pH7m34GW | University of Ghent collection | https://gateway.psb.ugent.be |
pH-35S::PIF3-ECFP-HA | This paper | N/A |
pH-pPIF3::PIF3-ECFP-HA | This paper | N/A |
pEarleyGate201 | Earley et al., 2006 | N/A |
pEarleyGate202 | Earley et al., 2006 | N/A |
pEG201-PIF3 | This paper | N/A |
pEG201-PIF5 | This paper | N/A |
pEG201-GFP | This paper | N/A |
pEG202-PIF1 | This paper | N/A |
pEG202-PIF3 | This paper | N/A |
pEG202-PIF4 | This paper | N/A |
pEG202-PIF5 | This paper | N/A |
pEG201-GI | This paper | N/A |
pEG201-GFP | This paper | N/A |
pGreenII 0800-LUC | Hellens et al., 2005 | N/A |
pGLUCpPIL1 | This paper | N/A |
pGLUCpCCA1 | This paper | N/A |
Software and Algorithms | ||
VisionWorksLS | UVP, LLC | N/A |
NIH ImageJ software | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
CFX Manager | BioRad | Cat#1845000 |
LabView | National Instruments | N/A |
BioDare2 | Moore et al., 2014; Zielinski et al., 2014 | https://www.biodare2.ed.ac.uk |
Bowtie (v1.0.0) | Langmead et al., 2009 | http://bowtie-bio.sourceforge.net/index.shtml |
MACS2 (v 2.1.1.) | Zhang et al., 2008 | https://pypi.org/project/MACS2/ |
NGSplot (v 2.41.4) | Shen et al., 2014 | https://github.com/shenlab-sinai/ngsplot |
HOMER | Heinz et al., 2010 | http://homer.ucsd.edu/homer/download.html |
Phaser | Mockler et al., 2007 | http://phaser.mocklerlab.org/ |
PANTHER | Thomas et al., 2003 | http://www.pantherdb.org/ |
R | RCoreTeam, 2016 | http://www.R-project.org/ |
ggplot2 plotting package | Wickham, 2009 | https://cran.r-project.org/web/packages/ggplot2/ |