Figure 3. DCs treated with NP(OVA+aVD₃) are impaired in both MHC class II-restricted exogenous antigen presentation and allogeneic T cell stimulatory capacity. (A) Immature DCs generated from bone marrow cells of BALB/c mice were treated with NP(OVA), NP(1,800:1), NP(600:1), or NP(200:1) (50 μg/ml as OVA) for 2 h. After washing and fixing, DCs were co-cultured with OVA_323−339-specific DOBW cells. The supernatants were harvested, and IL-2 production was measured by ELISA. Data are presented as the mean±SD of 3 independent experiments. (B) Immature DCs generated from C57BL/6 mouse bone marrow cells were treated with NP(OVA), NP(1,800:1), NP(600:1), or NP(200:1) (10 μg/ml as OVA) for 48 h. DCs were then co-cultured with T cells isolated from the spleens of BALB/c mice at the indicated ratios for 96 h. T cell proliferation was measured by the incorporation of (³H)-thymidine added during the final 18 h of culture.

^**p<0.01.