Figure 4. DCs treated with NP(OVA+aVD₃) induce Foxp3⁺ Tregs from naïve CD4⁺CD25⁻ T cells in allogeneic MLR. (A) Immature DCs generated from the bone marrow cells of C57BL/6 mice were treated with NP(OVA), NP(1,800:1), NP(600:1), or NP(200:1) (10 μg/ml as OVA) for 48 h. DCs were then co-cultured with CD4⁺CD25⁻ T cells isolated from the spleens of BALB/c mice at a ratio of 1:10 in a medium containing recombinant human IL-2 (100 U/ml) for 4 days. Cells were gated on CD4⁺ cells, and the expression levels of CD25 and Foxp3 were analyzed. The data show representative histograms of 3 independent experiments. (B) The proportion of CD25⁺FoxP3⁺ Tregs in the CD4⁺ cell population of each experimental group is shown. Data are presented as the mean±SD of 3 independent experiments.

^*p<0.05, ^**p<0.01.