Fig. 6. Embryonic developmental competence in type I diabetes mellitus mouse models was significantly lower compared with normal control mice. (A and B) Percentages of cleavage and blastulation after in vitro fertilization (IVF). The numbers of two-cell embryos and blastocysts were counted to compare pre-implantation embryonic development (n=85). (C and D) Total and apoptotic blastomeres of individual blastocysts were also evaluated by confocal microscopy (n=21). (E) Representative image of a hatching blastocyst after staining with 2-(4-amidinophenyl)-1H-indole-6-carboxamidine (DAPI) and terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL). Magnification was ×200 and the images were obtained by confocal microscopy. Blue and Green signal respectively indicate nucleus and apoptotic DNA of blastomeres. Different letters (a, b) indicate statistically significant differences (p<0.05). STZ, streptozotocin; CON, control; MII, metaphase II.