Fig. 2. Knockdown of NEAT1 attenuates Aβ-induced neuronal damage in SH-SY5Y and SK-N-SH cells. (A and B) The expression of NEAT1 was measured in SH-SY5Y and SK-N-SH cells transfected with si-NEAT1 or si-NC after treatment of 10 µM Aβ for 24 h by qRT-PCR. Cell viability (C and D), Ki67 expression (E and F), and apoptosis (G and H) were detected in SH-SY5Y and SK-N-SH cells transfected with si-NEAT1 or si-NC after treatment of 10 µM Aβ for 24 h by MTT, immunocytochemistry, and flow cytometry. ^*p<0.05, ^**p<0.01, ^***p<0.001. NEAT1, nuclear enriched abundant transcript 1; Aβ, amyloid β_1–42.