Table 1.
Summary of P. falciparum infection across a range of temperatures, showing the number of replicate infectious feeds, the number of mosquitoes dissected to examine for oocysts and sporozoites, the day post-blood-feed when sporozoites were first observed in mosquito salivary glands, and the equivalent EIP predicted by the Detinova degree-day model [9].
| mosquito species and temperature regime (°C) | no. of replicate infectious feeds | dissection days post feed (and total no. mosquitoes dissected for oocysts, and sporozoites) | presence of oocysts (and maximum prevalence on a given day) | presence of sporozoites (and maximum prevalence on a given day) | day sporozoites first detected in salivary glands | EIP (days) predicted by degree-day model |
|---|---|---|---|---|---|---|
| An. stephensi | ||||||
| 16 ± 0 | 3 | 40–62 (50, 92) | no | no | n.a. | infinite |
| 17 ± 0 | 2 | 34–60 (64, 273) | yes (55%) | yes (57%) | 38 | 111 |
| 18 ± 0 | 3 | 16–62 (402, 322) | yes (75%) | yes (73%) | 33 | 56 |
| 20 ± 0 | 1 | 11–39 (148, 114) | yes (80%) | yes (78%) | 26 | 28 |
| 14 ± 5 | 3 | 17–60 (144, 217) | yes (3%) | no | n.a. | infinite |
| 16 ± 5 | 6 | 16–55 (362, 377) | yes (5%) | yes (5%) | 30 | infinite |
| 18 ± 5 | 2 | 15–62 (286, 228) | yes (79%) | yes (85%) | 27 | 56 |
| An. gambiae | ||||||
| 17 ± 0 | 2 | 34–50 (107, 273) | yes (13%) | yes (3%) | 43 | 111 |
| 19 ± 0 | 2 | 23–41 (52, 383) | yes (40%) | yes (23%) | 29 | 37 |
| 19 ± 5 | 2 | 18–40 (80, 491) | yes (50%) | yes (33%) | 25 | 37 |