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. 2019 Jun 27;9:9324. doi: 10.1038/s41598-019-45796-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Firing rate histograms from representative recordings of the local effects of PHA-543613 and MLA (A,B), and PNU-120596 and NS-1738 (C) on the spontaneous and NMDA-evoked firing activity of CA1 hippocampal pyramidal neurons. Furthermore, panel (A) shows the time windows for calculating spontaneous and NMDA-evoked firing rates before (Sp, NMDA, respectively), and during (Sp_Drug, NMDA_Drug) microiontophoretic application of a given test compound. Insets to the right show a typical extracellular action potential recorded in the given experimental session. Bottom inset on panel (C) shows the raw electrophysiological recording in the time window marked on the horizontal axis of the corresponding histogram.