Fig. 3.

Analysis of drug efflux capability via ABC transporters. a Doxorubicin (Dox) fluorescence or phase contrast images of PaLung, WI-38, and MEF cells. Cells were pre-treated without or with 5 μM verapamil (Vera) for 30 min before the treatment with 10 μM doxorubicin alone or together with Vera for an additional 3 h. Images were acquired with 20× objective lens, and scale bar represents 50 μm. b Flow cytometry analyses of Dox accumulation in PaLung, PaKiT03, WI-38, HEK293T, and MEF cells. Cells were pre-treated without or with 5 μM verapamil (Vera, top row) or 5 μM cyclosporin A (CSA, bottom row) for 30 min before the treatment with 10 μM doxorubicin alone or together with Vera or CSA for an additional 3 h. c Flow cytometry analyses of Dox accumulation in cells derived from different tissues of P. alecto in the presence or absence of verapamil. Cells were treated and analysed as in (b). All results shown are representative of at least three experimental repeats