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. 2019 Jun 27;9:9339. doi: 10.1038/s41598-019-45411-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of ZNF518B knocking-down on the proliferation of DLD1 and HCT116 cells. (a) Efficiency of knocking-down for isoforms 1 and 2 in DLD1 and HCT116 cells after 48 h of transfection. (b) Influence of knocking-down on cell proliferation as measured by MTT assays; continuous lines, control cells; broken lines, cells transfected with ZNF518B siRNA. (c) Effects of ZNF518B knocking-down in a representative colony formation assay. (d) Quantification of the different cell colony formation assays. Statistical analysis of data in a and d was carried out with the Mann-Whitney test, whereas two-way ANOVA was used for data in panel b. (***p < 0.001).