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. 2019 Jun 27;9:9339. doi: 10.1038/s41598-019-45411-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Epigenetic modification of histones in the promoter and proximal coding region of the ZNF518B gene. The experiments were carried out at single-nucleosome level by using the Nuc-ChIP approach. (A) Acetylation marks in nucleosomes N − 3, N − 2 and N − 1. (B) Methylation marks at amplicon 75, which covers the 5′ part of nucleosome N + 1 in DLD1 and D-Mut1 cells and the 3′ part of the nucleosome covering the TSS in SW48 cells. Results were compared with the Kruskal-Wallis test. (**p < 0.005; ***p < 0.001).