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. 2019 Jun 27;10:2828. doi: 10.1038/s41467-019-10773-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A modified form of H2A.Z is required to regulate flowering time. a Western blot (WB) analysis of HTA9 levels in histone enriched extracts of wild-type (WT) and hta9hta11 mutants at 7 days after germination (DAG; upper panel). The same blot was probed with anti-H4 antibody for loading control (bottom panel). Arrows indicate the bands recognized by the antibody. b Schematic representation of the C-terminal region of FLAG-tagged HTA9 native construct (FLAG-HTA9_N) or mutated in which K129 or both K129 and K132 were replaced by R (FLAG-HTA9_RK and FLAG-HTA9_RR, respectively). c WB analysis of HTA9 and FLAG-HTA9 levels in WT, hta9hta11, WT/FLAG-HTA9_N, WT/FLAG-HTA9_RK, and WT/FLAG-HTA9_RR (upper panel). Arrows indicate the bands recognized by the antibody. The same blot was probed with anti-H4 antibody for loading control (bottom panel). d Picture showing WT, hta9hta11, hta9hta11/FLAG-HTA9_N, hta9hta11/FLAG-HTA9_RK, and hta9hta11/FLAG-HTA9_RR plants at 24 DAG growing under long day conditions. Arrows indicate the presence of elongated shoot. Bar indicates 1 cm. e Box plots indicating the number of rosette leaves at bolting in different genotypes. “n” indicates the number of plants. In each case, the median (segment inside rectangle), upper and lower quartiles (boxes), and minimum and maximum values (whiskers) are indicated. p Value of significant differences as determined by Student’s t test are indicated. f Relative FT expression levels in the different genotypes at 12 DAG. ACTIN2 (ACT2) was used as an internal control. Error bars indicate standard deviation of n = 4 biological replicates. Data points are indicated in the bar charts. g Relative FLC expression levels in different genotypes at 12 DAG. ACT2 was used as an internal control. Error bars indicate standard deviation of n = 4 biological replicates. Data points are indicated in the bar charts. Significant differences as determined by Student’s t test are indicated (**P < 0.01; *P < 0.05; ns, not significant). Source data of Fig. 1a, c, e–g are provided as a Source Data file