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. 2019 Jun 27;10:2828. doi: 10.1038/s41467-019-10773-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — H2A.Zub repressive effect is independent of Polycomb Repressive Complex 2 activity. a Western blot showing global H3K27me3 levels in wild-type (WT) and hta9hta11 mutants at 7 days after germination (DAG). The same blot was probed with anti-H4 antibody for loading control. Bottom bar chart shows quantification of H3K27me3 levels relative to H4. Error bars indicate standard deviation of n = 4 independent experiments. Data points are indicated in the bar charts. No significant (ns) differences between WT and hta9hta11 were found according to Student’s t test. b Left panel, Venn diagram showing the percentage of upregulated genes in hta9hta11 and atbmi1a/b weak that were enriched in H2A.Z and H3K27me3 marked in WT (H2A.Z/H3K27me3_WT) at 7 DAG. Right panel, Venn diagram showing the overlap between genes downregulated in hta9hta11 and H2A.Z/H3K27me3_WT genes. c H3K27me3 levels analyzed by chromatin immunoprecipitation–quantitative polymerase chain reaction at ATG surrounding region of several upregulated and downregulated genes in hta9hta11 that were H2A.Z/H3K27me3_WT (genes from overlaps of b). The levels were normalized to ACTIN7 (ACT7). Error bars indicate standard deviation of n = 3 biological replicates. Data points are indicated in the bar charts. No significant (ns) differences between WT and hta9hta11 were found according to Student’s t test. d Venn diagram showing the overlap between the H2A.Z-enriched genes that are upregulated in hta9hta11 and the ones upregulated in fie mutants. Source data of Fig. 5a, c are provided as a Source Data file