Table 2.

Primers, which were used for amplification and sequencing of cytb gene in mole voles of the Ellobius subgenus. Primers Eta_CytbF1, and VOLE14 were used to amplify the full cytb gene with flanked fragments of mtDNA; all other primers correspond to various internal areas of cytb gene, the position of their 5’-end nucleotide from the start of cytb gene is in parentheses.

Species	Primer designation	Nucleotide sequence of primer (5’–3’) and its localization within the full gene cytb	Citation
E. talpinus	Forward primers
	Eta_CytbF1	GAAACACCTAATGACAATCATACG	Bogdanov et al. 2015
	L15095-Ell	(370)-ATAGCCACAGCATTCATA	Bogdanov et al. 2015
	L15473-Ell	(748)-CTCGGAGACCCAGATAACTAC	Bogdanov et al. 2015
	Reverse primers
	MVZ04m	(431)-GTGGCCCCTCAAAATGATATTTGTCCTC	Bogdanov et al. 2015
	CLETH16m	(824)-AGGAAGTACCATTCTGGTTTAAT	Bogdanov et al. 2015
	VOLE14	TTTCATTACTGGTTTACAAGAC	Conroy and Cook 1999
E.tancrei, E.alaicus	Forward primers
	Eta_CytbF1	GAAACACCTAATGACAATCATACG	Bogdanov et al. 2015
	L15095-Ell	(370)-ATAGCCACAGCATTCATA	Bogdanov et al. 2015
	Vole23m	(590)-TCCTGTTCCTTCACGAAACAGGTTC	Bogdanov et al. 2015
	L15473-Elal	(748)-CTTGGAGACCCAGACAATTTC	Our design
	Reverse primers
	MVZ04m	(431)-GTGGCCCCTCAAAATGATATTTGTCCTC	Bogdanov et al. 2015
	CLETH16m	(824)-AGGAAGTACCATTCTGGTTTAAT	Bogdanov et al. 2015
	VOLE14	TTTCATTACTGGTTTACAAGAC	Conroy, Cook 1999