Table 3.

Primers, which were used for amplification and sequencing of XIST and Rspo1 genes in the mole voles of the Ellobius subgenus.

Nuclear gene	Primer designation	Nucleotide sequence of primer (5’–3’)	Source
XIST	Xist1-L11841	GGGGTCTCTGGGAACATTTT	Our design
	Xist1-R12504 or Xist1-Rint	TGCAATAACTCACAAAACCAAC AAGCAGGTAAGTATCCACAGC	Our design
Rspo1	Primers used for first amplification
	Rspo1F-Ell	CACTGTACACTTCCGGGTCTCTTT	Our design
	Rspo1R-Ell	AGAAGTCAACGGCTGCCTCAAGTG	Our design
	Primers used for second PCR with a PRISM®BigDye TM Terminator v. 3.1 kit
	Rspo1-5intF-Ell	CAGGCACGCACACTAGGTTGTAA	Our design
	Rspo1-1intR-Ell	GTCTAGACTCCCAACACCTG	Our design