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. 2019 Jun 27;9:9320. doi: 10.1038/s41598-019-45818-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sub-cellular distribution of mature miRNAs after chemical inhibition of the Wnt/β-catenin signalling pathway in HL-1 cells. (A) Schematic representation of cellular treatment and sub-cellular fractionation. (B) Control of fractionation purity by qRT-PCR (snU6 and Gapdh) and Western blot (cropped images of parts of the same gel) analysis (GAPDH and H3). (C) qRT-PCR for miR-133a and miR-1 levels in total cell lysates and purified nuclear fractions. (D) Representative FISH image of HL-1 cells co-stained for miR-133a (red) and DAPI (blue) for nuclei visualization (n = 5); *p < 0.05; **p < 0.01.