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. 2019 Jun 27;9:9320. doi: 10.1038/s41598-019-45818-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The nuclear enrichment of miR-133a in the Wnt off-state is dependent on the interaction between AGO2 and IPO8. (A) qRT-PCR for Ipo8 mRNA after transient transfection of specific siRNAs. (B) qRT-PCR for mature miR-133a in isolated nuclei of HL-1 cells treated as indicated. (C) Representative FISH image of HL-1 cells transfected with siRNAs and treated or not with IWR-1 as indicated. Red, miR-133a; blue, DAPI (D) Graphic plot of average miR-133a/nuclear area in HL-1 cells transfected with siRNAs and treated or not with IWR-1. (E) IPO8-AGO2 affinity evaluated in BRET assay in HL-1 cells transfected with AGO2 NanoLuc and IPO8-Halo and treated as indicated (n = 3); *p < 0.05; **p < 0.01; ***p < 0.001.