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. 2019 Jun 27;9:9313. doi: 10.1038/s41598-019-45752-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Granulomatous nephritis in olive ridleys. (a) Extensive fibrosis in the kidney of stranded turtle; note variably sized caseous nodules (granulomas) on cut surface (arrow). (b) Renal granuloma (arrowhead); note core of necrotic material surrounded by macrophages, including multinucleated giant cells, and a broad zone of fibrosis. There is secondary dilation of the surrounding renal tubules (asterisks). (c) Heterophils infiltrate multiple renal tubules (arrowheads). There is detachment of the renal epithelium and other artifacts resulting from autolysis. (d) Numerous gram-negative bacilli (arrowhead) within areas of nephritis from which S. Typhimurium was isolated. (e) Immunohistochemistry staining of renal granuloma with anti-Salmonella antibodies; note brown reactivity at the center of granulomas. (f) Positive control liver from a cattle egret with multifocal necrosis from which S. Typhimurium was cultured; note S. Typhimurium-positive foci (arrow). Inset: Pure culture of S. Typhimurium bacteria staining positive with anti-S. Typhimurium antibody.