Fig. 1.

Human GSTpi, but not mite GSTmu, increased the cysteine-protease activity of Der p 1 allergen. The proteolysis of the fluorogenic peptide (Boc-QAR-AMC, 75 μM) by Der p 1 was measured in the presence of different concentration of GSTpi or GSTmu (0–2.5 μM), after preactivation with (A) GSH or (B) l-Cys (range from 0.1 mM to 2 mM), for 90 min. Data are expressed as the μM of 7-amino-4-methylcoumarin (AMC) released by Der p 1 and showed as means ± SD of duplicate measurements. A representative example of two independent experiments is shown.