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. 2019 Jun 11;26:101254. doi: 10.1016/j.redox.2019.101254

Fig. 5.

Fig. 5

Contrast media induced PINK1-Parkin–mediated mitophagy, NLRP3 inflammasome activation, and apoptosis in HK-2 cells. HK-2 cells are incubated in DMEM/F12 containing iohexol (20 mg I/ml) for 72 h. (A, B) Immunoblot analysis and quantification of MFN1 and DRP1. (C, D) Representative images and quantification of immunofluorescence double-labelling LC3B and mitochondrial marker (MitoTracker). Scale bar: 10 μm. (E, F) Representative TEM images of mitochondrial morphology and mitophagosomes (red arrow). Data were shown as a dot plot of the number of mitophagosomes from 10 images of each group. Scale bar: 500 nm. (G, H) Immunoblot analysis of PINK, Parkin, SQSTM1, LC3B I/II, and COX IV. (I–L) Representative images and quantification of immunofluorescence double-labelling PINK1/Parkin and MitoTracker. (M-P) Immunoblot analysis and quantification of NLRP3, caspase-1 p20, IL-1β p17, cleaved caspase-3, Bax, and Bcl-2. Data were presented as mean ± TEM. *p < 0.05, **p < 0.01, ***p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)