Fig. 7.

Silencing PINK1 or PARK2 decreased mitophagy in contrast-treated HK-2 cells. HK-2 cells are subjected to iohexol (20 mg I/ml) for 72 h at 24 h after transfection with negative control siRNA (SiNC), PINK1 siRNA (SiPINK1), or PARK2 siRNA (SiPARK2). (A, B) Immunoblot analysis of PINK1, Parkin, SQSTM1, LC3B I/II, and COX IV. (C, D) Representative images and quantification of immunofluorescence double-labelling LC3B and MitoTracker in SiNC, SiPINK1, or SiPARK2 cells treated with iohexol. Scale bar: 50 μm. (E, F) Representative images and quantification of double-labelling LC3B and Parkin. Scale bar: 50 μm. Data were presented as mean ± TEM. *p < 0.05, **p < 0.01, ***p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)