Figure 2.

Cell lysates of E. coli with (+) or without (−) exogenous Hsp33 (16% (w/w)) were treated with plasma for the indicated amount of time. Insoluble proteins were separated from soluble proteins by centrifugation and both fractions were subjected to SDS-PAGE (a,b) and Bradford analysis (c). (a) Soluble protein remaining in the supernatant after plasma treatment. (b) Insoluble protein in the pellet fraction. ^†High molecular mass protein aggregates; *the band to the left corresponds to added Hsp33. The data shown are representative of four independent experiments. (c) Bradford quantitation of the protein fractions. Blue bars (left) represent samples without added Hsp33, green bars (right) those to which 16% (w/w) Hsp33 was added prior to plasma treatment. Solid bars (dark): soluble proteins, shaded bars (light): insoluble fraction. For each time point, the total amount of protein (soluble plus insoluble) was set to 100%. The data reflect the results of three independent experiments and standard deviation. (Online version in colour.)