Figure 4.
Investigation of the reversibility of plasma activation of Hsp33. (a) Inactivation kinetics of Hsp33P60 and Hsp33P120 incubated with DTT and ZnCl2. (b) Activity (blue, left) and thiol oxidation status (green, right) of Hsp33 in the chaperone assay initially (red (abbreviation for reduced)), after activation with HOCl or plasma for 45 s (P45), inactivation by reduction (HOCl-red or P45-red, respectively), and a second activation by HOCl (HOCl-red-HOCl) or plasma (P45-red-P45). The data reflect at least three independent experiments. (c,d) CD spectra of Hsp33 after activation, inactivation and reactivation with HOCl (c) or plasma (d). (Online version in colour.)