Figure 5.

Influence of ROS scavengers GSH and DTT on Hsp33 activity and kinetics of scavenger oxidation by plasma. (a) Relative chaperone activity of Hsp33 after DBD plasma treatment in the absence or presence of GSH (10 or 20 mM) or DTT (5 or 10 mM). Data reflect three independent experiments. (b) Free thiols in 25 µM DTT, 50 µM GSH, or 50 µM cysteine after different plasma treatment times (compare to Hsp33 thiol oxidation in figure 3b). Insert: For DTT, GSH, or cysteine, free thiol quantitation for the untreated controls (P0) and the 60 s plasma treatment time point (P60) is shown with the data reflecting three independent experiments. (Online version in colour.)