Table 2.

Key advantages and disadvantages of TCO-Tz Click PEGylation.

Advantage

Disadvantage

•Hypothesis-driven. •Can identify reversible oxidation occupancy and type. •Catalyst-free. •Selective and bio-orthogonal. •Internally normalised. •Flexible—method can be readily adapted. •Democratic—makes use of readily available equipment and techniques. •Cost and time efficient. •Suitable as an orthogonal workflow to redox proteomics. •Could be useful for studying the redox state of hydrophobic and/or difficult to digest proteins.

•Investigator bias given hypothesis driven nature. •Relies on the antibody recognising the PEGylated protein. •Biophysical interaction between SDS and PEG. •Careful PEG, antibody and gel size selection is required. •May be difficult to study proteins with many solvent exposed thiols. •Inability to use technique in certain species when available epitopes are not conserved. •Multiple bands preclude multiplexing.