Table 1.
Summary of the multiple sequencing datasets in this study.
| Sequencing Samples | Bases (Gbp) | Read (×106) | Clean rare | >Q20 | >Q30 | GC content | Mean coverage |
|---|---|---|---|---|---|---|---|
| BGISEQ500-WES | 29.41 | 294.30 | 0.41% | 96.72% | 89.14% | 49.75% | 328.49X |
| MGISEQ2000-WES | 16.34 | 163.55 | 0.25% | 98.18% | 92.08% | 49.71% | 129.40X |
| HiSeq4000-WES | 41.93 | 283.70 | 4.46% | 97.36% | 93.01% | 50.63% | 395.17X |
| NovaSeq-WES | 25.88 | 178.87 | 2.25% | 95.33% | 92.67% | 49.73% | 241.52X |
| BGISEQ500-WGS | 126.86 | 1270.02 | 1.76% | 93.73% | 83.33% | 41.76% | 41.03X |
| MGISEQ2000-WGS | 137.36 | 1374.87 | 0.21% | 96.17% | 88.19% | 41.76% | 45.13X |
| HiSeq4000-WGS | 191.00 | 1276.10 | 8.25% | 95.90% | 90.11% | 41.69% | 58.00X |
| NovaSeq-WGS | 98.30 | 657.45 | 1.28% | 95.89% | 93.86% | 41.61% | 28.96X |
| HiSeq Xten-WGS | 134.00 | 894.58 | 7.29% | 94.50% | 87.63% | 40.71% | 38.93X |
The sequencing datasets was generated from multiple sequencers in both BGI and Illumina platforms. The length of reads in each of BGI and Illumina platforms were PE100 and PE150, respectively. The clean data rare indicated that the percentage of reads with low quality trimmed by fastp. “>Q20/Q30 percentage” indicates the percent of bases with quality score (−10*lg (error rate)) higher than 20 and 30 (indicating error rates of 1% and 1‰, respectively). The Mean sequence Depth indicates the average sequencing depth of datasets.