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. 2019 Jun 27;9:9326. doi: 10.1038/s41598-019-45740-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell differentiation from human HAP stem-cell colonies produced from cultured cryopreserved hair follicles. HAP stem cell colonies differentiated to nestin and βIII-tubulin-positive neurons, GFAP-positive glial cells, K15-positive keratinocytes, SMA-positive smooth muscle cells, and cTnT-positive cardiac muscle cells. Staining for nestin, βIII-tubulin, GFAP, K15, SMA, and cTnT is shown in red color in corresponding panels. DAPI is shown in blue color in each panel. Scale bar = 100 µm.