Figure 4.

Proposed dual glycolytic pathways in C. bescii. The conventional pathway is shown in green where oxidation of NADH and reduced ferredoxin leads to H₂ production via the bifurcating hydrogenase. The alternative GOR-dependent pathway and oxidation of the resulting reduced ferredoxin by the Ech hydrogenase and the associated ATP synthase is shown in dark yellow. Abbreviations: HK, hexokinase; PGI, phosphoglucose isomerase; PFK, phosphofructokinase; FBA, fructose-bisphosphate aldolase; BFH₂ase, bifurcating [FeFe] hydrogenase; PGM, phosphoglycerate mutase; ENO, enolase; PK, pyruvate kinase; Fd, ferredoxin; POR, pyruvate ferredoxin oxidoreductase; LDH, lactate dehydrogenase; PTA, phosphotransacetylase; ACK, acetate kinase.