Figure 2.

LC-MS–based activity assays of UGT76E1, UGT76E2, UGT7E11, UGT76E12, and UGT74F1 with 12-OH-JA. Activity tests of 10 μg of purified UGT76E1, UGT76E2, UGT76E11, UGT76E12, and UGT74F1 were performed with 0.1 mm 12-OH-JA and 0.5 mm UDP-Glc for 1 h at 25 °C. The reactions were stopped by adding 25 μl of acetonitrile and analyzed by LC-MS. Extracted ion chromatograms are shown for the product 12-O-Glc-JA ([M-H]⁻ 387.116). Signal intensity is given in counts per second (cps). Data represent a single experiment.