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. 2019 Jun 28;39(6):BSR20190066. doi: 10.1042/BSR20190066

Figure 1. MiR-876-5p up-regulation suppressed GC cell viability and migration, but induced cell apoptosis.

Figure 1

(A) The five acknowledged miRNAs (miR-5195-3p, miR-3666, miR-23c, miR-4429 and miR-876-5p) were chosen to qRT-PCR. (B) MiR-876-5p expression was overexpressed in MGC803 and MKN-45 cells. (C,D) GC cell proliferation was measured by CCK-8 and EdU assays. (E) Caspase-3 activity was detected to evaluate the GC cell apoptosis in response to miR-876-5p overexpression. (F) Apoptotic cell ratio in MGC803 and MKN-45 was detected by flow cytometric analysis. (G) Western blot assay was used to assess the level of apoptosis-related protein (Bcl-2 and Bax). (H) Transwell migration assay was conducted in MGC803 and MKN-45 cells. (I) The alteration of EMT-associated protein (E-cadherin and N-cadherin) level was analyzed by Western blotting. (J) Immunofluorescence staining examined the different expression levels of EMT biomarkers in MGC803 transfected with miR-NC or miR-876-5p mimics. **P < 0.01 and *P < 0.05 vs control group.