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. 2019 Jun 28;39(6):BSR20190066. doi: 10.1042/BSR20190066

Figure 5. MiR-876-5p inhibited GC cellular process via targeting WNT5A and MITF.

Figure 5

(A) GC cell proliferative capacity was assessed by CCK-8 assay in MGC803 cell after transfection with miR-NC, miR-876-5p mimics, miR-876-5p mimics + WNT5A or miR-876-5p mimics + MITF. (B,C) Caspase-3 activity and flow cytometry assays indicated that the positive impact of miR-876-5p mimics on GC cell apoptosis was reversed by pcDNA-WNT5A and pcDNA-MITF. (D) The level alteration of Bcl-2 and Bax after treatment with various transfection plasmids. (E) The effect of the enhanced WNT5A and MITF expressions on GC cell migration in miR-876-5p-up-regulated MGC803 cell. (F) EMT process was promoted by miR-876-5p overexpression, but this acceleration was weakened by overexpression of WNT5A and MITF. (G) Immunofluorescence staining examined the different expression levels of EMT biomarkers in MGC803 cells transfected with miR-NC, miR-876-5p mimics, miR-876-5p mimics + WNT5A or miR-876-5p mimics + MITF. **P < 0.01 and *P < 0.05 vs control group.