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. 2019 Jun 28;39(6):BSR20190857. doi: 10.1042/BSR20190857

Figure 3. Effect of higenamine on the expressions of iNOS and COX-2 in IL-1β-stimulated NPCs.

Figure 3

NPCs were pretreated with 10, 20, 40 μM of higenamine for 2 h and then treated with IL-1β (10 ng/ml) for 24 h. (A,B) The mRNA expression levels of iNOS and COX-2 were determined using quantitative real-time PCR (qRT-PCR). (C) The expressions of two inflammatory mediators, iNOS and COX-2, were measured using Western blot analysis. (D,E) Quantification analysis of iNOS and COX-2. *P < 0.05 vs. control NPCs. #P < 0.05 vs. IL-1β-stimulated NPCs.