Figure 2.

Effect of the different buckwheat (BW) products investigated on colon myofibroblasts migration in absence (A,B) or presence (C,D) of the pro-inflammatory cytokine TNF-α (20 ng/mL) for 48 h of exposure. The histograms display the ability of the myofibroblasts to migrate as percent of scratched area covered. The cells corresponding to the histograms A and B were treated with 10 or 50 μL, respectively, of the digested-BW products and control digesta (Ct). The histograms C and D were also treated with 10 or 50 μL, respectively, of the digested-BW products and control digesta in the presence or absence (Ct) of TNF-α (20 ng/mL). The average ± SD from 3 – 5 different experiments are showed. * indicates statistically significant differences (p < 0.05) from Ct (A,B) and TNF-α-treated cells (C,D). Abbreviations: Ct: control digesta; _bBW: bread baked from “BIO” wheat flour and roasted BW flour (50/50%); _b500BW: bread baked from wheat flour type 500 and roasted BW flour (50/50%); BWg: roasted BW groats; _fBWg: ‘Tempeh’ type product from dehulled roasted BW; BWs₁₀₀: solution obtained from the digestion of 100 mg of BW sprouts; BWs₅₀: solution obtained from the digestion of 50 mg of BW sprouts.