Fig.2.

SDS-PAGE analysis of tau aggregates. A) Tau was subjected to sedimentation assay, followed by the analysis of supernatant and pellet in comparison with the total. At 0 h, full-length tau does not form higher molecular weight aggregates. But in the presence of limonoids, these higher molecular weight species are observed, which were more prominent in presence of nimbin. B) At 24 h of incubation, tau exhibits the formation of aggregates as majority of tau was getting separated in pellet. Moreover, similar observation was obtained in presence of salannin. In case of nimbin, tau exhibited partial aggregation as equal amounts of tau was observed in supernatant and pellet. C) Later at 120 h, tau exhibited maximal aggregation which was witnessed by the presence of higher order species in pellet. Nimbin and salannin also showed maximal aggregation, where salannin led to complete aggregation of tau.