Fig.3.

Biocompatibility of limonoids. A) 10⁴ cells/well were seeded in 96 well plate and incubated with 0.05 and 50μM concentrations of limonoids at 37°C 5% CO₂ for 24 h, followed by MTT assay and calculation of % cell viability. Both nimbin and salannin have lesser cytotoxic effect and may be potent as drug candidate. B) HEK293T cells were seeded in 96 well plate and co-treated with 10μM human tau aggregates and then with different concentrations (1, 2, and 5μM) of limonoids separately and incubated for 24 h at 37°C 5% CO₂. Cell viability was determined by MTT assay where nimbin (2μM) were more effective to reduce tau aggregates mediated toxicity at lower concentration than salannin (5μM) in HEK293T cells. The significance was calculated using SigmaPlot and ***, ** and * indicates p value <0.001, <0.01 and <0.05 respectively. ns is not significant where p≥0.05.