Fig.2.

α-Syn pathology is preferentially exacerbated along the nigrostriatal tract. A) Microphotographs of α-syn pathology (pS129-immunoreactive cell bodies and neurites) in the substantia nigra of WT and En1^+/– animals unilaterally injected with PFFs at one month of age. All microphotograph data are representative images from one animal from each respective cohort. B) Stereological estimates of pS129⁺-labeled cells in the substantia nigra ipsilateral and contralateral to the site of PFF injection (N≥8). No α-syn pathology across any stereologically evaluated regions was observed in PBS-treated animals. C) Stereological estimates of TH⁺ labeled cells in the substantia nigra in both WT and En1^+/– mice injected unilaterally with either PBS or PFFs. D) Corresponding densitometric quantification of TH-immunoreactive striatal integration (N≥5). Results are depicted as mean±SEM. pS129+ and TH+ cell count data were analyzed by negative binomial mixed-effects regression with separate analyses performed to compare pathology ipsilateral to injection and contralateral to injection. Striatal TH⁺ optical density data were analyzed by linear mixed-effects regression. Statistics: all ^*p < 0.05, ^**p < 0.01. Scale bar: A – 250μm.