Fig.3.

Representative photomicrographs showing the pattern of both AT100 and pS396 immunostaining in cases with low (I−III) (A) or high (V−VI) (B) Braak Stages. White arrows mark examples of neurons presenting no colocalization, whereas yellow arrows mark double-labeled neurons. (C) Histograms show the AT100/pS396 labeling ratio (left) or the colocalization percentage (right) in all the cases examined in this study grouped by low or high Braak Stage. A paired t test found significant differences in the comparisons between low and high Braak stages for both AT100/S396 ratio and colocalization (mean ± SD). ^***p < 0.0005. Scale bar: 60 μm.