Fig. 1.

Impaired PSII proteostasis alters the chloroplast proteome in the Arabidopsis var2 mutant. (A) Schematic representation of MS-based analysis of the total chloroplast proteins isolated from 3-week-old plants of var2 and the WT grown under continuous light (80 µmol m^–2 s^–1) at 20±2 °C. (B) Distribution of 1474 proteins detected in either var2 or the wild-type (WT) (see Supplementary Table S2). (C) Label-free quantitation identified a total of 603 differentially accumulated proteins (Log₂ FC ≥ 1; P<0.05, Student’s t-test), among which, 317 were increased and 286 were reduced in var2 as compared to the WT (Supplementary Tables S3, S5).