Fig. 6.

Trp-oxidized photosynthetic proteins are significantly accumulated in the Arabidopsis var2 mutant. (A) Steady-state levels photosynthetic proteins and GAPA-2 in var2 and the wild-type (WT). All proteins except HCF136 and PsaH exhibited at least 1.8-fold higher levels in var2. The data represent mean intensity values of the related peptides (n=3 replicates) (Supplementary Table S2). (B) Oxidation levels of the proteins. These proteins exhibited at least 1.6-fold higher oxidation levels in var2 as compared with the WT. The levels of oxidation were calculated using total intensities of peptides carrying oxidized Trp residues (Supplementary Table S8). The data represent the means of n=3 replicates. GAPA-2, a non-photosynthetic protein, was also found to undergo Trp-oxidation but showed no change in expression or in oxidation levels in var2 as compared with the WT.