Fig. 3.

Transcriptome analysis of Aethionema TUR and CYP seeds by RNA-Seq. (A–C) Number of differentially expressed genes and Venn diagrams showing the proportion of overlapping genes in pairwise comparisons between TUR and CYP, and between exposure to darkness (D, grey seed icon), or white light (WL, yellow icon). Icons showing germinating seeds indicate the capability of samples for germination; note that the seeds had not yet germinated at the point of sampling. (D) Heatmap indicating the relative expression of the overlapping 87 genes in the four treatments. Coloring is based on reads per kilobase of transcript per million mapped reads (RPKM) values. A detailed list is provided in Supplementary Fig. S1. (E) Gene list of three selected GO terms with average RPKM values and relative expression differences, indicated by shades of blue (lowest RPKM value) or red (highest RPKM value).