Fig. 6.

Phenotypic rescue of the Arabidopsis gl3 egl3 tt8 triple-mutant by mutant AtGL3 protein variants. The triple-mutant was transformed with 35S::AtGL3, 35S::AtGL3 (F177I), and 35S::AtGL3 (D477G) and analysed in the T1 generation. The top row shows seedlings grown on half-strength Murashige and Skoog medium with 4% sugar under Basta selection at 4 d after germination. Scale bars are 2 mm. Representative images are shown and the proportions of rescued plants/Basta-resistant plants are indicated below. The bottom row shows rosette leaves of 2-week-old T1 plants that had previously been analysed for the anthocyanidin phenotype at 9 d after germination. The proportion of plants showing trichome rescue in the plants that exhibited anthocyanidin rescue are indicated below. Scale bars are 5 mm.