Table 2.

Complementary biofilm extraction conditions tested (by order of harshness increment).

Condition no.	Description
1	Intact biomass without treatment (control)
2	Biomass was treated with 1X lysis buffer (Promega) for 40 min at RT
3	Biomass was treated with 0.01 mg/mL lysozyme in 1X lysis buffer (Promega) for 30 min at 37°C
4	Cells were treated with 1 mg/mL lysozyme in buffer (0.1 M tris-HCl + 0.05 M ethylenediaminetetraacetic acid (EDTA), pH 8.0) for 30 min at 37°C. Then, the biomass was sonicated in a Branson Digital Sonifier 250 (United States) with 3/16″ exponential horn for 15 min at 50% amplitude (229 μm) and a frequency of 20 kHz
5	Cells were treated with 2 mg/mL lysozyme in buffer (0.1 M tris-HCl + 0.05 M EDTA, pH 8.0) for 30 min at 37°C. Then, the biomass was precipitated and the pellet resuspended in 5X lysis buffer (Promega) and incubated for 40 min at RT. Then, the biomass was sonicated in a Branson Digital Sonifier 250 (United States) with 3/16″ exponential horn for 15 min at 50% amplitude (229 μm) and a frequency of 20 kHz in the presence of glass beads