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. 2019 Mar 27;67(7):523–535. doi: 10.1369/0022155419841013

Figure 1.

Figure 1.

Morphological characterization of primary cilia on differentiated and undifferentiated mouse cochlear precursor hair cells. (A) Representative images showing double-immunocytochemical staining for Ac-tubulin (red) and γ-tubulin (green) in the undifferentiated US/VOT-E36 cells. Representative scanning electron microscopy (SEM) (B) and transmission electron microscopy (TEM) (C, D) of the undifferentiated cells. (E, F) Images showing double-immunocytostaining for Ac-tubulin (red) and γ-tubulin (green) in the undifferentiated cells (incubated at 33C) and differentiated cells (incubated at 39C). Scale bars = 5 μm (A), 1 μm (B, C), 0.5 μm (D), 10 μm (E, F). Abbreviation: US/VOT-E36, University of Sheffield/ventral otocyst-epithelial cell line clone 36.